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KMID : 0613820160260010068
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Journal of Life Science
2016 Volume.26 No. 1 p.68 ~ p.74
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Analysis of a Sulfur-oxidizing Perchlorate-degrading Microbial Community
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Kim Young-Hwa
Han Kyoung-Rim
Hwang Hee-Jae
Kwon Hyuk-Jun
Kim Ye-Rim
Kim Kwon-Woo
Kim Hee-Joo
Son Myung-Hwa
Choi Young-Ik
Sung Nak-Chang
Ahn Yeong-Hee
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Abstract
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Perchlorate (ClO4-) is an emerging pollutant detected in surface water, soil, and groundwater. Previous studies provided experimental evidence of autotrophic ClO4- removal with elemental sulfur (S0) particles and activated sludge, which are inexpensive and easily available, respectively. In addition, ClO4- removal efficiency was shown to increase when an enrichment culture was used as an inoculum instead of activated sludge. PCR-DGGE was employed in the present study to investigate the microbial community in the enrichment culture that removed ClO4- autotrophically. Microorganisms in the enrichment culture showed 99.71% or more ClO4- removal efficiency after a 7-day incubation when the initial concentration was approximately 120 mg ClO4-/l. Genomic DNA was isolated from the enriched culture and its inoculum (activated sludge), and used for PCR-DGGE analysis of 16S rRNA genes. Microbial compositions of the enrichment culture and the activated sludge were different, as determined by their different DGGE profiles. The difference in DGGE banding patterns suggests that environmental conditions of the enrichment culture caused a change in the microbial community composition of the inoculated activated sludge. Dominant DGGE bands in the enrichment culture sample were affiliated with the classes β-Proteobacteria, Bacteroidetes, and Spirochaetes. Further investigation is warranted to reveal the metabolic roles of the dominant populations in the ClO4- degradation process, along with their isolation.
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KEYWORD
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Bacterial community, enrichment culture, elemental sulfur, perchlorate, PCR-DGGE
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